The complete mitochondrial genome of Polyozellus multiplex (Thelephorales)

Abstract The complete mitogenome of Polyozellus multiplex (Underw.) Murrill 1910, was first sequenced, assembled, and annotated in the present study. The mitogenome length was 47,054 bp with a GC content of 23.35%, including 14 conserved protein-coding genes, one ribosomal protein (RPS3), two DNA polymerases (DPO), two rRNA genes (RNS and RNL), and 24 transfer RNA (tRNA) genes. Phylogenetic analysis, based on a combined mitochondrial gene dataset from 17 taxa of four orders within the class Agaricomycetes, was conducted using maximum-likelihood (ML) and Bayesian inference (BI) methods. It is revealed that P. multiplex is closely related to Thelephora aurantiotincta Corner 1968, both of them have been clustered into Thelephorales.

Polyozellus multiplex (Underw.) Murrill. was originally described from Mount Desert, Maine, USA and classified in the genus Cantharellus Adans. ex Fr. 1821 with Cantharellus multiplex Underw. 1899 (Underwood 1899). Then, Murrill (1910) classified this fungus to a new genus, Polyozellus Murrill. 1910, as its cespitose imbricate habit differed from that of other species of Cantharellus. Imazeki (1953) reclassified this genus to the Thelephora Ehrh. ex Wild. 1787 group according to its lobed nodulose basidiospores thelephoric acid content, which differed from other Cantharellus species. Since then, Polyozellus has remained a monotypic genus. Voitk et al. (2017) (Yang 1992). Here, the mitochondrial genome of P. multiplex is first reported to promote our understanding in Polyozellus taxonomy and genetics.
Two genes (ITS1-5.8S-ITS2: OL913875, LSU rDNA: OL913876) were combined to identify P. multiplex, which was from the Dashaba Agriculture Market (104 5 0 48 00 E; 26 13 0 17 00 N), Xuanwei City, Yunnan, China. In accordance with guidelines and regulations provided by Kunming Institute of Botany, Chinese Academy of Sciences and the local government agency, the fungus collection from this local market was performed without a required ethical approval or other relevant permissions from a national or local agency. The dried specimen (voucher # HKAS122651) was deposited in the Herbarium of Cryptogams, Kunming Institute of Botany, Chinese Academy of Sciences (http:// www.kib.ac.cn/, the contact person and e-mail: Mr. Tao Deng, dengtao@mail.kib.ac.cn).
The total genomic DNA of this dried P. multiplex specimen was extracted using the CTAB method (Doyle 1987). The whole-genome sequencing was performed on an Illumina sequencing platform (HiSeq PE150) with standard procedures. The 150 bp paired-end libraries were prepared to generate approximately 8 GB of raw data. The mitochondrial genome was assembled by GetOrganelle v. 1.7.5 with the default parameters (Jin et al. 2020). The mitogenome was annotated by the MFannot tool (http://megasun.bch.umontreal.ca/cgibin/mfannot/mfannotInterface.pl) and was compared with the result annotated from the reference sequence Thelephora aurantiotincta with manual corrections in Geneious Prime 2020.0.3 (BioMatters, Ltd., Auckland, New Zealand).

Author contributions
Jian-Wei Liu and Fuqiang Yu designed the study, Jian-Wei Liu and Shi-Mei Yang analyzed the data. Jian-Wei Liu and Thatsanee Luangharn wrote, while Fuqiang Yu revised the manuscript. All authors agree to be accountable for all aspects of the work.

Disclosure statement
No potential conflicts of interest were reported by the authors.  Data availability statement